Technical Data

136779
Clone Type
Monoclonal
Host
Mouse
Source
Mouse
Isotype
IgG1
Clone Number
S87-25
Grade
Affinity Purified
Applications
IC IF IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-GABRB3 (GABAAR, Gamma-Aminobutyric Acid Receptor Subunit Beta-3, GABA(A) Receptor Subunit Beta-3)
GABAAR, Gamma-Aminobutyric Acid Receptor Subunit Beta-3, GABA(A) Receptor Subunit Beta-3

The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).

Applications
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1-10ug/ml Western Blot: 1ug/ml Immunohistochemistry: 0.1-1ug/ml Immunocytochemistry: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein aa370-433 of mouse GABA-A-R Beta3.
Form
Supplied as a liquid in PBS, pH 7.4, 50% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse GABRB3 at ~55kD. Species Crossreactivity: Human and rat. No cross-reactivity against GABA-A-R-Beta 2 or –Beta1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 4. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
USBio References
No references available
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