Technical Data

136784
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu
Gene ID
2932
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GSK3B (Glycogen Synthase Kinase-3 beta, GSK-3 beta, GSK3beta Isoform, Serine/Threonine-protein Kinase GSK3B)
Glycogen Synthase Kinase-3 beta, GSK-3 beta, GSK3beta Isoform, Serine/Threonine-protein Kinase GSK3B

Glycogen synthase kinase 3, is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues. It has also been shown to regulate immune and migratory processes, and is tied to pathways of cell proliferation and apoptosis (1-3). In mammals, it is encoded by two genes- GSK-3Alpha and GSK-3Beta. They are structurally similar, but functionally non-identical. GSK-3Alpha is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3Beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signaling (4). GSK-3 alpha and GSK-3 beta share 85% amino acid identity. GSK-3 has been implicated in a number of diseases including Type II diabetes, Alzheimer’s, cancer and bipolar (4, 5).

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human GSK-3Beta at C-terminal.
Form
Supplied as a liquid in PBS, pH 7.4.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GSK-3. Species Crossreactivity: mouse, rat, boine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Jope R.S. (2007) Neurochem Res. 32(4-5): 577-595. 2. Wang H. (2011) Cytokind. 53(2): 130-140.|3. Mills C.N. (2011) Front Mol Neurosci. 47(4): 47. 4. Hardt S.E., Sadoshima J. (2002) Circ Res. 90(10): 1055-1063. 5. Doble D.W., Woodgett J.R. (2003) J Cell Sci. 116(Pt7): 1175-1186.
USBio References
No references available
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