Technical Data

137460
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu Mk
Gene ID
23286
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-KIBRA (Kidney And Brain Protein)

The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of YAP (Yes-associated protein), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). Recent studies have identified KIBRA as a novel regulator of Hippo signaling (5-7). KIBRA has been shown to regulate Hippo signaling through its interaction with tumor suppressors Merlin (Mer) and Expanded (Ex) in Drosophila (7) and by associating with large tumor suppressors LATS1 and LATS2 in humans (8). In humans, KIBRA is predominantly expressed in the kidney and brain (9) and has been shown to play a role in hippocampus-related memory performance (10-12). Recent studies have shown that phosphorylation of KIBRA is highest during mitosis and is controlled by aurora kinase and protein phosphatase 1 (13).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Ala585 of human KIBRA.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of human KIBRA. Species Crossreactivity: monkey

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Pan, D. (2010) Dev Cell 19, 491-505. 2. Harvey, K.F. et al. (2003) Cell 114, 457-67. 3. Zhao, B. et al. (2010) Genes Dev 24, 862-74. 4. Zhao, B. et al. (2008) Curr Opin Cell Biol 20, 638-46. 5. Baumgartner, R. et al. (2010) Dev Cell 18, 309-16. 6. Genevet, A. et al. (2010) Dev Cell 18, 300-8. 7. Yu, J. et al. (2010) Dev Cell 18, 288-99. 8. Xiao, L. et al. (2011) J Biol Chem 286, 7788-96. 9. Kremerskothen, J. et al. (2003) Biochem Biophys Res Commun 300, 862-7. 10. Papassotiropoulos, A. et al. (2006) Science 314, 475-8. 11. Bates, T.C. et al. (2009) Neurosci Lett 458, 140-3. 12. Schaper, K. et al. (2008) Neurobiol Aging 29, 1123-5. 13. Xiao, L. et al. (2011) J Biol Chem 286, 36304-15.
USBio References
No references available
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