Technical Data

137466
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
13G47(D3K7B)
Grade
Purified
Applications
ChIP IP WB
Crossreactivity
Hu Mo Rt
Gene ID
9794
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MAML1 (Mastermind-like Protein 1)

Mastermind-like (MAML) family of proteins are homologs of Drosophila Mastermind. The family is composed of three members in mammals: MAML1, MAML2, and MAML3 (1,2). MAML proteins form complexes with the intracellular domain of Notch (ICN) and the transcription factor CSL (RBP-Jk) to regulate Notch target gene expression (3-5). MAML1 also interacts with myocyte enhancer factor 2C (MEF2C) to regulate myogenesis (6). MAML2 is frequently found to be fused with Mucoepidermoid carcinoma translocated gene 1 (MECT1, also know as WAMTP1 or TORC1) in patients with mucoepidermoid carcinomas and Warthin's tumors (7).

Applications
Suitable for use in ChIP, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 ChIP: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Asp269 of human MAML1.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified.
Specificity
Recognizes endogenous levels of total human MAML1. Species Crossreactivity: mouse, rat. Does not detect MAML2 or MAML3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Wu, L. et al. (2002) Mol. Cell Biol. 22, 7688-7700. 2. Lin, S.E. et al. (2002) J. Biol. Chem. 277, 50612-50620. 3. Kitagawa, M. et al. (2001) Mol. Cell Biol. 21, 4337-4346. 4. Nam, Y. et al. (2003) J. Biol. Chem. 278, 21232-21239. 5. Nam, Y. et al. (2006) Cell 124, 973-983. 6. Shen, H. et al. (2006) Genes Dev. 20, 675-688. 7. Tonon, G. et al. (2003) Nat. Genet. 33, 208-213.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net