Technical Data

137467
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
13G48(D3J4Y)
Grade
Purified
Applications
WB
Crossreactivity
Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MASTL (Microtubule-associated Serine/threonine Kinase-like)

Mitotic control is important for normal growth, development, and maintenance of all eukaryotic cells. Research studies have demonstrated that inappropriate control of mitosis can lead to genomic instability and cancer (reviewed in 1,2). A regulator of mitosis, Greatwall kinase (Gwl), was first identified in Drosophila melanogaster (3). Subsequent studies showed that, based on sequence homology and function, microtubule-associated serine/threonine kinase-like (MASTL) is the human ortholog of Gwl (4). Regulation of MASTL/Gwl activation has been shown to be critical for the correct timing of mitosis. Research studies have shown that Gwl is activated by hyperphosphorylation (5). The phosphorylation of human Gwl at Thr194 and Thr207 by active cyclin B1-cdc2 leads to possible autophosphorylation at Ser875 (Ser883 in Xenopus), which stabilizes the kinase. Activated Gwl phosphorylates alpha-Endosulfine (ENSA) and cAMP-regulated phosphoprotein 19 (ARPP19) at Ser67 and Ser62, respectively. Phosphorylated ENSA and ARPP19 inhibit the activity of the B55 subunit-associated form of protein phosphatase 2A (PP2A-B55), allowing for complete phosphorylation of mitotic substrates by cyclin B1-cdc2 and mitotic entry. When Gwl is inactivated, PP2A-B55 reactivates, which leads to dephosphorylation of cyclin B1-cdc2 and mitotic exit (5,6, reviewed in 7).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues near the N-terminal of human MASTL.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified.
Specificity
Recognizes endogenous levels of total human MASTL. Species Crossreactivity: mouse, rat, monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Eichhorn, P.J. et al. (2009) Biochim Biophys Acta 1795, 1-15. 2. Norbury, C. and Nurse, P. (1992) Annu Rev Biochem 61, 441-70. 3. Yu, J. et al. (2004) J Cell Biol 164, 487-92. 4. Voets, E. and Wolthuis, R.M. (2010) Cell Cycle 9, 3591-601. 5. Blake-Hodek, K.A. et al. (2012) Mol Cell Biol 32, 1337-53. 6. Vigneron, S. et al. (2011) Mol Cell Biol 31, 2262-75. 7. Lorca, T. and Castro, A. (2012) Oncogene 32, 537-543.
USBio References
No references available
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