Mouse Anti-FOLH1 (FOLH, NAALAD1, PSM, PSMA, Glutamate Carboxypeptidase 2, Cell Growth-inhibiting Gene 27 Protein, Folate Hydrolase 1, Folylpoly-gamma-glutamate Carboxypeptidase, Glutamate Carboxypeptidase II, Membrane Glutamate Carboxypeptidase, N-acetylated-alpha-linked Acidic Dipeptidase I, Prostate-specific Membrane Antigen, Pteroylpoly-gamma-glutamate Carboxypeptidase) (Biotin)
Prostate-specific membrane antigen (PSMA) is a 100-120kD type II transmembrane glycoprotein that is a glutamate-preferring carboxy-peptidase. Three functionally distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate. PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelial cells from breast, duodenum, and kidney tissues, and in prostate cancer.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:2000 Optimal dilutions to be determined by the researcher.
Hybridoma
F0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to aa117-351 from human PSMA expressed in E.coli.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PSMA.