Mouse Anti-Bcl-X (Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52))
Recognizes a protein of 27kD, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2a or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241aa protein which suppresses cell death. And Bcl-XS (short), a 178aa protein lacking a 63aa domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
Applications
Suitable for use in Flow Cytometry, Immunofluorescence, ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher.
Positive Control
Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa3-14 (Cys-QSNRELVVDFLS) from human Bcl-X.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138499-AF.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human Bcl-X, ~27kD. Species Crossreactivity: mouse, rat and porcine.
