Rabbit Anti-beta-Catenin (Cadherin associated protein, Beta 1 88kD, Catenin beta-1, CATNB, CHBCAT, CTNNB1)
Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Applications
Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RT Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher.
Positive Control
HeLa or MCF-7 cells. Breast carcinoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the middle of human beta-Catenin (p120).
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138503-AF.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human beta-catenin (p120). Species Crossreactivity: mouse.
