This monoclonal antibody recognizes the peptide core of gastric mucin M1 (>1,000kD) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epi thelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not w ith normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin r eactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fet al colonic) mucins.

Suitable for use in FLISA and Immunohistochemistry. Other applications not tested.

Immunohistochemistry (Formalin-fixed): Staining of formalin-fixed tissue requires boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20mins followed by coooling at RT for 20mins. Optimal dilutions to be determined by the researcher.

Stomach

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.

Purified by Protein G affinity chromatography.

Recognizes human MUC5AC. Species Crossreactivity: feline, chicken, hedgehog, monkey, mouse, porcine, rabbit, rat. Does not react with bovine.