138599-AF
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGApplications
FC IF IHC IP WBCrossreactivity
Bo Ca Eq Hu Mo RtAccession #
NP_000353Gene ID
7078Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Rabbit Anti-Tissue Inhibitor of Metalloproteinases 3 (TIMP Metallopeptidase Inhibitor 3, TIMP3, TIMP-3)
TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.
Applications
Suitable for use in Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RT Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher.
Positive Control
Placenta or breast carcinoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peptide corresponding to aa175-211 from TIMP3. Species Sequence Homology: mouse, rat
Form
Supplied as a liquid in PBS. Also available with BSA and azide, see 138599.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human TIMP3. Species Crossreactivity: mouse, rat, bovine, canine and equine.
