Rabbit Anti-UPK3A (Uroplakin-3A, UP3a, UPIIIa)
Uroplakin-3A (UPK3A) is a component of the asymmetric unit membrane (AUM). It is a highly specialized bio-membrane made by terminally differentiated urothelial cells. The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. UPK3A also contributes to the formation of urothelial glycocalyx, which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection . UPK3A has been shown as a helpful marker for the detection of bladder cancer.
Applications
Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher.
Positive Control
HEK2 93 cells or bladder carcinoma
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa260-279 (GASESSYTSVNRGPPLDRAE) from human UPK3A.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138601-AF.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human UPK3A.
