Technical Data

138814
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
13k4
Grade
Affinity Purified
Applications
IF IHC WB
Crossreactivity
Bo Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-DJ-1 (Oncogene DJ1, Parkinson Disease Protein 7, PARK7)

Protein DJ-1 has many roles including protecting cells against oxidative stress and cell death (Ref: SwissProt). Mutations in the DJ-1 gene have been associated with rare forms of autosomal recessive early-onset Parkinson's disease.

Applications
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:1000 Western Blot: 1:10,000 Immunohistochemistry: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Full length recombinant protein corresponding to human DJ-1 expressed in and purified from E. coli.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DJ-1, at ~21kD. Species Crossreactivity: mouse, bovine

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SM and Ariga H DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem. Biophys. Res. Commun. 231, 509–513 (1997). 2. Bonifati V, Rizzu P, van Baren, M.J., Schaap O, Breedveld GJ, Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M et al. Mutations in the DJ-1 gene associated with autosomal recessive early-onset Parkinsonism. Science, 299, 256–259 (2003). 3. Xu J, Zhong N, Wang H, Elias JE, Kim CY, Woldman I, Pifl C, Gygi SP, Geula C, Yankner BA. The Parkinson's disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Hum Mol Genet. 14(9):1231-41 (2005). 4. Yokota T, Sugawara K, Ito K, Takahashi R, Ariga H. And Mizusawa, H. Down regulation of DJ-1 enhances cell death by oxidative stress, ER stress, and proteasome inhibition. Biochem. Biophys. Res. Commun., 312, 1342–1348 (2003). 5. Taira T, Saito Y, Niki T, Iguchi-Ariga SM, Takahashi K and Ariga H. DJ-1 has a role in antioxidative stress to prevent cell death. EMBO Rep., 5, 213–218 (2004). 6. Bonifati V, Oostra BA. and Heutink, P. Linking DJ-1 to neurodegeneration offers novel insights for understanding the pathogenesis of Parkinson’s disease. J.Mol,Med.82,163-174 (2004).
USBio References
No references available
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