Rabbit Anti-CGRP (CALCA, CALC1, Calcitonin Gene-related Peptide 1, Alpha-type CGRP, Calcitonin Gene-related Peptide I)
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:100-1:200 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-100 Immunocytochemistry: 1:50-1:500 Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
MGHHHHHHSGSEF- MGFRKFSPFL ALSILVLYQA GSLQAAPFRS ALESSPDPAT LSKEDARLLL AALVQDYVQM KASELKQEQE TQGSSSAAQK RACNTATCVT HRLAGLLSRS GGMVKSNFVP TNVGSKAFGR RRRDLQA
Positive Control (Supplied)
139518A: Suppled as a recombinant protein (aa1-127) in loading buffer (100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa1-127 with a N-terminal His-Tag from human CGRP, expressed in E. coli
Form
Supplied as a liquid in PBS, pH 7.4, 0.01% sarcosyl, 1mM DTT, 5% trehalose, 0.05% Proclin 300
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes human Calcitonin Gene Related Peptide (CGRP)
