Rabbit Anti-Clara Cell Protein 16 (CC16, CC10, CCPBP, CCSP, Clara Cell Phospholipid-binding Protein, Clara Cells 10kD Secretory Protein, Secretoglobin Family 1A Member 1, UGB, UP1, UP-1, Urinary Protein 1, Urine Protein 1, Uteroglobin)
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:100-1:200 Western Blot: 1:100-1:400 Immunocytochemistry: 1:100-1:500 Immunohistochemistry (frozen): 1:100-1:500 Immunohistochemistry (paraffin sections): 1:50-1:200 Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
MGHHHHHHSGSEF - DICPGFLQV LEALLLGSES NYEAALKPFN PASDLQNAGT QLKRLVDTLP QETRINIVKL TEKILTSPLC EQDLRV
Positive Control Included
139736AA: Clara Cell Protein 16, Positive Control Suppled as a recombinant protein (aa22-96) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol. DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa22-96 with a N-terminal His-Tag from rat CC16, expressed in E. coli
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
Purity
Purified by affinity chromatography
Specificity
Recognizes rat Clara Cell Protein 16 (CC16)