Rabbit Anti-GDF15 (Growth/Differentiation Factor 15, GDF-15, Placental Bone Morphogenetic Protein, Placental TGF-beta, Macrophage Inhibitory Cytokine 1, MIC-1, Prostate Differentiation Factor, NSAID-activated Gene 1 Protein, NAG-1, NSAID-regulated Gene 1 Protein, NRG-1, MIC1, PDF, PLAB, PTGFB)
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 0.05-2ug/ml Western Blot: 0.5-2ug/ml Immunohistochemistry (frozen): 5-20ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control Included
140555A: GDF15, Positive Control Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to His193-Val296 from mouse GDF15, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse GDF15.
