Rabbit Anti-Lymphocyte Activation Gene 3 (LAG3, CD223)
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5-2ug/ml Immunocytochemistry: 5-20ug/ml Immunohistochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control (Supplied)
141309A: Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 0.02% BPB, 50mM DTT, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to Thr244-Glu487 from human LAG3, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human Lymphocyte Activation Gene 3 (LAG3)
