Rabbit Anti-Trefoil Factor 2 (TFF2, SML1, SP, Spasmolytic Protein 1)
Human spasmolytic polypeptide (HSP) is a trefoil peptide found prominently in the foveolar and surface epithelium, pyloric glands and mucous neck cells of the stomach. This antibody will help detect HSP in normal and uncharacterized tissues.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:100-200 Western Blot: 1:100-400 Immunocytochemistry: 1:100-500 Immunohistochemistry: 1:100-500 (frozen) Immunohistochemistry: 1:50-200 (paraffin sections) Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
TFF2 (Ser27~Tyr129), MGHHHHHHSGSEF-SPCR CSRMTPSNRK NCGFPGITSD QCFNLGCCFD SSVAGVPWCF HPLPNQASEQ CVMEVSAREN CGYPGISPED CASRHCCFSN LIFEVPWCFF PQSVDDCHY
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat Trefoil Factor 2 (TFF2)
