Mouse Anti-eEF2 (Eukaryotic translation elongation factor 2, EF2, EEF-2, EF-2, elongation factor 2, polypeptidyl-tRNA translocase)
(Eukaryotic translation elongation factor 2, EF2, EEF-2, EF-2, elongation factor 2, polypeptidyl-tRNA translocase)
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant human EEF2 protein fragments expressed in E. coli.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.2% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of EEF2. Does not cross-react with related proteins. Species Crossreactivity: human
