Technical Data

143178
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
13k51
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Gene #
EGFP
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Green Fluorescent Protein, Enhanced (EGFP, EYFP)

The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:5000-1:20,000 Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length purified recombinant EGFP, expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.3, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human Enhanced Green Fluorescent Protein (EGFP).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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