Mouse Anti-LRRFIP1 (GC-binding factor 2 TAR RNA-interacting protein)
(GC-binding factor 2 TAR RNA-interacting protein)
Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5´-AGCCCCCGGCG-3´) and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant human LRRFIP1 protein fragments expressed in E. coli.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.2% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of LRRFIP1. Does not cross-react with related proteins. Species Crossreactivity: human, mouse
