Mouse Anti-PCNA (Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, Pcna/cyclin, PCNA_HUMAN, PCNAR, Polymerase delta accessory protein, Proliferating cell nuclear antigen)
(Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, PCNA, Pcna/cyclin, PCNA_HUMAN, PCNAR, Polymerase delta accessory protein, Proliferating cell nuclear antigen)
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunocytochemistry: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant human PCNA protein fragments expressed in E. coli.
Form
Supplied as a liquid in ascites fluid.
Specificity
Recognizes endogenous levels of PCNA. Does not cross-react with related proteins. Species Crossreactivity: human, mouse, monkey, rat, hamster