Technical Data

144435
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
CK-7
Grade
Ascites
Applications
IHC WB
Crossreactivity
Hu
Gene ID
KRT7
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C
Mouse Anti-Cytokeratin Peptide 7 (SCL, cytokeratin 7, K2C7, K7, keratin, Keratin-7, 55K type II cytoskeletal, keratin, type II cytoskeletal 7, sarcolectin, , , KERATIN, SIMPLE EPITHELIAL, KERATIN, TYPE II, CYTOSKELETAL, 7, SARCOLECTIN, Type-II keratin Kb7)

KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.

UniProt Number
Human(P08729), Mouse(Q9DCV7), Rat(Q6IG12)
Gene ID
KRT7
Applications
Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen).
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Handling
Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.
Form
Ascites, supplied as a lyophilized powder. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Specificity
Recognizes human Cytokeratin Peptide 7. No crossreactivity with other proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Rosenberg, M.; Fuchs, E.; Le Beau, M. M.; Eddy, R. L.; Shows, T. B. : Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12. Cytogenet. Cell Genet. 57: 33-38, 1991.|2. Smith, F. J. D.; Porter, R. M.; Corden, L. D.; Lunny, D. P.; Lane, E. B.; McLean, W. H. I. : Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse. Biochem. Biophys. Res. Commun. 297: 818-827, 2002.
USBio References
No references available
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