144441-HRP
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
HRPIsotype
IgGGrade
Affinity PurifiedApplications
E IC IHC WBCrossreactivity
HuGene ID
DDTShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Rabbit Anti-DDT (D-dopachrome tautomerase, Phenylpyruvate tautomerase II) (HRP)
DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Handling
Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
E. coli-derived human DDT recombinant protein (Position: M1-L118).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DDT. No crossreactivity with other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Esumi, N., Budarf, M., Ciccarelli, L., Sellinger, B., Kozak, C. A., Wistow, G.Conserved gene structure and genomic linkage for D-dopachrome tautomerase (DDT) and MIF.Mammalian Genome 9: 753-757, 1998.|2. Nishihira, J., Fujinaga, M., Kuriyama, T., Suzuki, M., Sugimoto, H., Nakagawa, A., Tanaka, I., Sakai, M.Molecular cloning of human D-dopachrome tautomerase cDNA: N-terminal proline is essential for enzyme activation.Biochem. Biophys. Res. Commun. 243: 538-544, 1998.USBio References
No references available