144565
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
HuGene ID
GBP1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-GBP1 (GTP-binding protein 1)
Interferons induce a large number of genes in their target cells, including those coding for guanylate-binding proteins (GBPs). GBPs, such as GBP1, are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3 . The 593-amino acid GBP1 protein shares 77% and 88% identity with GBP2 and GBP3 , respectively. All GBPs, including GBP1, have a conserved N-terminal globular GTP-binding domain containing 2 consensus sequences and a third T(L/V)RD sequence not found in other GTPases. The GBP1 gene to the GBP gene cluster on chromosome 1p22.2. It is located telomeric to GBP2 and centromeric to GBP3. High expression of GBP1, GBP2, and GBP3 are in endothelial cells after stimulation with IFNG , TNF , or IL1B .
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of human GBP1.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitutie with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GBP1. No crossreactivity with other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Cheng, Y.-S. E., Patterson, C. E., Staeheli, P.Interferon-induced guanylate-binding proteins lack an N(T)KXD consensus motif and bind GMP in addition to GDP and GTP.Molec. Cell. Biol. 11: 4717-4725, 1991.|2. Olszewski, M. A., Gray, J., Vestal, D. J.In silico genomic analysis of the human and murine guanylate-binding protein (GBP) gene clusters.J. Interferon Cytokine Res. 26: 328-352, 2006.|3. Tripal, P., Bauer, M., Naschberger, E., Mortinger, T., Hohenadl, C., Cornali, E., Thurau, M., Sturzl, M.Unique features of different members of the human guanylate-binding protein family.J. Interferon Cytokine Res. 27: 44-52, 2007|USBio References
No references available