Technical Data

144604
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Mo Rt
Gene ID
GSTP1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GSTP1 (GST class-pi, GSTP1-1)

Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.

UniProt Number
P09211
Gene ID
GSTP1
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide mapping at the N-terminal of human GSTpi, different from the related rat and mouse sequences by two amino acids.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitutie with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human, mouse and rat GSTP1. No crossreactivity with other proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Morrow, C. S.; Cowan, K. H.; Goldsmith, M. E. : Structure of the human genomic glutathione S-transferase-pi gene. Gene 75: 3-11, 1989|2.Islam, M. Q.; Platz, A.; Szpirer, J.; Szpirer, C.; Levan, G.; Mannervik, B. : Chromosomal localization of human glutathione transferase genes of classes alpha, mu and pi. Hum. Genet. 82: 338-342, 1989. |3.Konohana, A.; Konohana, I.; Schroeder, W. T.; O'Brien, W. R.; Amagai, M.; Greer, J.; Shimizu, N.; Gammon, W. R.; Siciliano, M. J.; Duvic, M. :Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. J. Invest. Derm. 95: 119-126, 1990. |
USBio References
No references available
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