Technical Data

144607
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Gene ID
ABCB4
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ABCB4 (MDR3, PGY3, GBD1, MDR2, PFIC-3, P-Glycoprotein 3, Multidrug resistance protein 3, ATP-binding cassette sub-family B member 4, P-glycoprotein 3)

Adenosine triphosphate-binding cassette subfamily B, member 4 (ABCB4), also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. MDR1 and MDR3 constitute the human P-glycoprotein gene family. The MDR3 gene contains 28 exons and 27 of these contain coding sequences for the two homologous halves of the protein that correlate with functional domains. MDR3 gene mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.

UniProt Number
P21439
Gene ID
ABCB4
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Handling
Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa1-20, MDLEAAKNGTAWRPTSAEGD at the N-terminal of human ABCB4.
Form
Supplied as a lyophilized powder from PBS, 4% trehalose. Reconstitute with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ABCB4. No crossreactivity with other proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lincke, C. R., Smit, J. J., van der Velde Koerts, T., Borst, P. Structure of the human MDR3 gene and physical mapping of the human MDR locus. J. Biol. Chem. 266: 5303-5310, 1991. 2. Rosmorduc, O., Hermelin, B., Poupon, R. MDR3 gene defect in adults with symptomatic intrahepatic and gallbladder cholesterol cholelithiasis. Gastroenterology 120: 1459-1467, 2001
USBio References
No references available
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