144791
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
IHC WBCrossreactivity
Hu Mo RtGene ID
ITGB2Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-ITGB2/CD18 (Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Complement receptor C3 subunit beta,CD18)
ITGB2(INTEGRIN, BETA-2)，also known as CD18, is a protein that in humans is encoded by the ITGB2 gene.ITGB2 is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18.The ITGB2 gene is mapped to 21q22.3. The expression of CD18 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of ITGB2 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although ITGB2 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of human ITGB2, identical to the related mouse sequence, different from the related rat sequence by one amino acid.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitutie with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human, mouse and rat ITGB2/CD18. No crossreactivity with other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Abramson, J. S., Mills, E. L., Sawyer, M. K., Regelmann, W. R., Nelson, J. D., Quie, P. G. Recurrent infections and delayed separation of the umbilical cord in an infant with abnormal phagocytic cell locomotion and oxidative response during particle phagocytosis. J. Pediat. 99: 887-894, 1981. |2. Akao, Y., Utsumi, K. R., Naito, K., Ueda, R., Takahashi, T., Yamada, K. Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1 beta subunit. Somat. Cell Molec. Genet. 13: 273-278, 1987. |3. "Entrez Gene: ITGB2 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)".|USBio References
No references available