Technical Data

144900-ML550
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
MaxLight™550
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Gene ID
MIF
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Rabbit Anti-MIF (Glycosylation-inhibiting factor) (MaxLight 550)

Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2 MIF plays a critical role in inflammatory diseases and atherogenesis.

UniProt Number
P14174
Gene ID
MIF
Applications
Suitable for use in FLISA, Western Blot.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
E. coli-derived human MIF recombinant protein (Position: M1-A115).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MIF. No crossreactivity with other proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bernhagen, J.; Krohn, R.; Lue, H.; Gregory, J. L.; Zernecke, A.; Koenen, R. R.; Dewor, M.; Georgiev, I.; Schober, A.; Leng, L.; Kooistra, T.; Fingerle-Rowson, G.; Ghezzi, P.; Kleemann, R.; McColl, S. R.; Bucala, R.; Hickey, M. J.; Weber, C. : MIF is a noncognate ligand of CXC chemokine receptors in inflammatory and atherogenic cell recruitment. Nature Med. 13: 587-596, 2007.|2. Bozza, M.; Kolakowski, L. F., Jr.; Jenkins, N. A.; Gilbert, D. J.; Copeland, N. G.; David, J. R.; Gerard, C. : Structural characterization and chromosomal location of the mouse macrophage migration inhibitory factor gene and pseudogenes. Genomics 27: 412-419, 1995|3. Budarf, M.; McDonald, T.; Sellinger, B.; Kozak, C.; Graham, C.; Wistow, G. : Localization of the human gene for macrophage migration inhibitory factor (MIF) to chromosome 22q11.2. Genomics 39: 235-236, 1997.
USBio References
No references available
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