Technical Data

145001
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Gene ID
NTRK2
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C
Rabbit Anti-NTRK2 (Trk-B, GP145-TrkB, TrkB tyrosine kinase, Tropomyosin-related kinase B, Neurotrophic tyrosine kinase receptor type 2)

Neurotrophic tyrosine kinase, receptor, type 2 , also known as BDNF/NT-3 growth factors receptor or TrkB receptor, is a protein that in humans is encoded by the NTRK2 gene. The TrkB receptor is part of the large family of receptor tyrosine kinases. This gene is mapped to 9q21.33. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for this gene.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Handling
Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to a sequence at the N-terminus of human TrkB, aa70-90, EIFIANQKRLEIINEDDVEAY), different from the related mouse and rat sequences by 1aa.
Form
Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 200ul of ddH2O to 0.5mg/ml.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NTRK2. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Berghuis, P., Dobszay, M. B., Wang, X., Spano, S., Ledda, F., Sousa, K. M., Schulte, G., Ernfors, P., Mackie, K., Paratcha, G., Hurd, Y. L., Harkany, T. Endocannabinoids regulate interneuron migration and morphogenesis by transactivating the TrkB receptor. Proc. Nat. Acad. Sci. 102: 19115-19120, 2005. 2. Luberg, K., Wong, J., Weickert, C. S., Timmusk, T. Human TrkB gene: novel alterative transcripts, protein isoforms and expression pattern in the prefrontal cerebral cortex during postnatal development. J. Neurochem. 113: 952-964, 2010. 3. Nikoletopoulou, V., Lickert, H., Frade, J. M., Rencurel, C., Giallonardo, P., Zhang, L., Bibel, M., Barde, Y.-A. Neurotrophin receptors TrkA and TrkC cause neuronal death whereas TrkB does not. Nature 467: 59-63, 2010.
USBio References
No references available
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