145095
Clone Type
PolyclonalHost
RabbitIsotype
IgGGrade
Affinity PurifiedApplications
IHC WBCrossreactivity
Hu RtGene ID
PRLRShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Prolactin receptor (PRLR)
PRLR (Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al. (1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al. (1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues (histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR (conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1-0.5ug/ml Immunohistochemistry (Paraffin): 0.5-1ug/ml. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutes is required for staining of formalin/parffin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa591-605, QLGRLDYLDPTCFMH at the C-Terminal of mouse PRLR The sequence is identical to the related rat sequence, and different from the related human sequence by three amino acids. Species Sequence Homology: mouse.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul dH2O.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes PRLR. Species Crossreactivity: human and rat. No crossreactivity with other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Arden, K. C., Boutin, J.-M., Djiane, J., Kelly, P. A., Cavenee, W. K. The receptors for prolactin and growth hormone are localized in the same region of human chromosome 5. Cytogenet. Cell Genet. 53: 161-165, 1990. 2. Arden, K. C., Cavenee, W. K., Boutin, J.-M., Kelly, P. A. The genes encoding the receptors for prolactin and growth hormone map to human chromosome 5. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A129 only, 1989. 3. Cunningham, B. C., Bass, S., Fuh, G., Wells, J. A. Zinc mediation of the binding of human growth hormone to the human prolactin receptor. Science 250: 1709-1712, 1990.USBio References
No references available