145127
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
Hu Mo RtGene ID
RGS14Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-RGS14 (regulator of G-protein signalling 14)
Regulator of G-protein signaling 14, also called RGS14, is a protein that in humans is encoded by the RGS14 gene. This gene encodes a member of the regulator of G-protein signaling family. By genomic sequence analysis, this gene is mapped to chromosome 5q35.3. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of human RGS14, identical to the related mouse and rat sequences.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitutie with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human, mouse and rat RGS14. No crossreactivity with other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Kimple, R. J., Kimple, M. E., Betts, L., Sondek, J., Siderovski, D. P. Structural determinants for GoLoco-induced inhibition of nucleotide release by G-alpha subunits. Nature 416: 878-881, 2002.|2. Traver, S., Bidot, C., Spassky, N., Baltauss, T., de Tand, M.-F., Thomas, J.-L., Zalc, B., Janoueix-Lerosey, I., de Gunzburg, J. RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of G-alpha-0. Biochem. J. 350: 19-29, 2000.|USBio References
No references available