145287-ML550
Clone Type
PolyclonalHost
RabbitSource
MouseConjugate
MaxLight™550Isotype
IgGGrade
Affinity PurifiedApplications
FLISA IHC IP N WBCrossreactivity
MoGene ID
TNFShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Rabbit Anti-TNF alpha (Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2) (MaxLight 550)
TNFα(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Applications
Suitable for use in FLISA, Western Blot, Immunohistochemistry (Paraffin), Neutralization and Immunoprecipitation.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
E. coli-derived mouse TNF alpha recombinant protein (Position: L80-L235).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse TNF alpha. No crossreactivity with other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., Wallace, R. B.Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor.Nature 313: 803-806, 1985.|2. Pennica, D., Nedwin, G. E., Hayflick, J. S., Seeburg, P. H., Derynck, R., Palladino, M. A., Kohr, W. J., Aggarwal, B. B., Goeddel, D. V.Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin.Nature 312: 724-729, 1984.USBio References
No references available