145293
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
HuGene ID
TNFRSF10AShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-TNFRSF10A (Death receptor 4, TNF-related apoptosis-inducing ligand receptor 1)
TNFRSF10A (Tumor Necrosis Factor Receptor Subfamily Member 10A), also known as APO2, DR4 or TRAILR1, is a protein that in humans is encoded by the TNFRSF10A gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. By analysis of radiation hybrids, Marsters et al. (1997) mapped the DR4 gene to 8p21. The TRAIL receptor DR5, and 2 decoy receptors for TRAIL, DCR1, and DCR2, are located in the same region, suggesting that these receptors arose from recent gene duplication events. Pan et al. (1997) found that, as with FAS, TNFR1, and DR3, overexpression of DR4 induced apoptosis. However, unlike the other 3 death receptors, DR4 did not use FADD to transmit the death signal, suggesting the use of distinct proximal signaling machinery.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminal of human TNFRSF10A.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitutie with 200ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TNFRSF10A. No crossreactivity with other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Marsters, S. A., Sheridan, J. P., Pitti, R. M., Huang, A., Skubatch, M., Baldwin, D., Yuan, J., Gurney, A., Goddard, A. D., Godowski, P., Ashkenazi, A. A novel receptor for Apo2L/TRAIL contains a truncated death domain. Curr. Biol. 7: 1003-1006, 1997. |2. Pan, G., O'Rourke, K., Chinnaiyan, A. M., Gentz, R., Ebner, R., Ni, J., Dixit, V. M. The receptor for the cytotoxic ligand TRAIL. Science 276: 111-113, 1997.|USBio References
No references available