Technical Data

145321
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Isotype
IgG1
Clone Number
TM-33
Grade
Ascites
Applications
WB
Crossreactivity
Ch Hu Mo Rt
Gene ID
TPM1
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C
Mouse Anti-Tropomyosin (36/39kD) (CMH3, TMSA, CMD1Y, C15orf13, HTM-alpha, Alpha-tropomyosin, Tropomyosin-1)

The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.

UniProt Number
Human(P09493), Mouse(P58771), Rat(P04692)
Gene ID
TPM1
Applications
Suitable for use in Western Blot.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Handling
Store at -20˚C for one year. After reconstitution, store at 4˚C for one month. Can also be aliquoted and stored frozen at -20˚C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Chicken gizzard tropomyosin.
Form
Ascites, supplied as a lyophilized powder. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Specificity
Recognizes human, mouse, rat and chicken Tropomyosin (36/39 kDa). No crossreactivity with other proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lees-Miller, J. P.; Helfman, D. M. : The molecular basis for tropomyosin isoform diversity. BioEssays 13: 429-437, 1991.|2. Gariboldi, M.; Manenti, G.; Dragani, T. A.; Pierotti, M. A. : Chromosome mapping of nine tropomyosin-related sequences in mice. Mammalian Genome 6: 273-277, 1995.||
USBio References
No references available
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