Technical Data

145384
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
APC
Isotype
IgG2b
Clone Number
810506
Grade
Affinity Purified
Applications
FC FLISA
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-ALK-7 (Activin Receptor-like Kinase 7, Activin RIC, ACVR1C, ACVRLK7) (APC)

Transforming Growth Factor beta (TGF-B) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes consisting of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various Smad proteins. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-B superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors [also termed activin receptor like kinase (ALK)] and five type II receptors have been isolated from mammals. ALK2, 3, 4, 5, and 6 are also known as Activin R1A, BMPR1A, Activin R1B, TGF-B R1, and BMPR1B, respectively, reflecting their ligand preferences. ALK7 contains a cysteine-rich domain with conserved cysteine spacing in the extracellular region that is shared by other type I receptors, and a glycine-and-serine-rich domain (the GS domain) preceding the kinase domain.

Applications
Suitable for use in Flow Cytometry and FLISA. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul labels 1x10e6 PC-3 human prostate cancer cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Recombinant protein corresponding to Leu26-Glu113 from human ALK-7, expressed in CHO cells.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant
Specificity
Recognizes human ALK-7. Does not react with rat ALK-7.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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