Mouse Anti-Arhgef9 (Collybistin, KIAA0424, PEM-2, Cdc42 Guanine Nucleotide Exchange Factor (GEF) 9)
Collybistin, also known as ARHGEF9, is an ~60kD cytoplasmic guanine nucleotide exchange factor. It is expressed in the brain and is upregulated at the onset of neuronal differentiation. Collybistin associates with Neuroligin2, Gephyrin, and GABA receptor subunits to form a complex which is required for GABAergic synaptogenesis as well as hippocampal synaptic plasticity. Mutations in Collybistin are associated with a variety of neurological disorders including epilepsy, anxiety, aggression, mental retardation, and hyperplexia. Human Collybistin contains an SH3 domain (aa867), a DH domain (aa103287), and a Pleckstrin homology domain (aa318426). Within aa390516, human Collybistin shares 97% and 57% aa sequence identity with mouse and rat Collybistin, respectively.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 2ug/ml Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of human brain (medulla) Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Asn390-Lys516 from human Collybistin/ARHGEF9, expressed in E. coli.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Collybistin/ARHGEF9.
