Technical Data

145393
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
APC
Isotype
IgG2a
Clone Number
175128
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Rat Anti-Axl (Ark, Ufo) (APC)

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of receptor tyrosine kinases.1, 2 The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis.3 This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul, 1x10e6 cells Optimal dilutions to be determined by the researcher.
Hybridoma
Mouse myeloma cells with spleen cells from Balb/c rats.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light.
Immunogen
Recombinant protein corresponding to aa19-445 from mouse Axl, NS0-derived (UniProt # Q00993).
Form
Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A or G affinity chromatography.
Specificity
Recognizes mouse Axl.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465. 2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022. 3. Holland, S. et al. (2005) Canc. Res. 65:9294.
USBio References
No references available
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