Mouse Anti-LRRC3B (Leucine Rich Repeat Containing 3B, LRP15)
LRRC3B, also known as LRP15, is a transmembrane leucine-rich protein that is downregulated by promoter methylation in gastric and colorectal cancers. It confers resistance to ultraviolet light-induced DNA damage and functions as a tumor suppressor. Mature human LRRC3B contains an LRR N-terminal domain (aa34-64), 3 LRR repeats (aa65-135), an LRR C-terminal domain (aa145-197), a transmembrane segment (aa205-225), and a C-terminal region (aa226-259). Within aa28-205, human LRRC3B shares 100% aa sequence identity with mouse and rat LRRC3B.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa28-205 from human LRRC3B, CHO-derived (UniProt # Q96PB8).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A or G affinity chromatography.
Specificity
Recognizes human LRRC3B in direct ELISAs and Western blots.
