Rabbit Anti-NKIRAS2 (KBRAS2, NF-kappa-B Inhibitor-interacting Ras-like Protein 2, I-kappa-B-interacting Ras-like Protein 2, Kappa B-Ras Protein 2, KappaB-Ras2, DKFZp434N1526, DKFZP434N1526, MGC74742)
KappaB ras-1 (kB-ras-1) and kB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IkappaB (IkB), an inhibitor of the transcription factor NF-kB. IkB exists in two homologous forms, IkB-a and IkB-B, although IkB-B contains a unique 47aa region within its ankyrin domain. While inactive IkB-a-NF-kB complexes can shuttle in and out of the nucleus, IkB-B-NF-kB complexes are retained exclusively in the cytoplasm. It is suggested that kB-ras proteins preferentially bind to the IkB-B form through this unique insert within the ankyrin region, thus modulating the cellular location of IkB-B and regulating the rate of degradation of IkB-B. This antibody is specific for kB-ras2 and has no cross-reactivity to kB-ras1.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
RAW264.7 Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 14aa from near the C-terminus of human KappaB ras2.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human KappaB ras2. Species Crossreactivity: mouse and rat.
