146105
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IF IHC WBCrossreactivity
Hu MoShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-BCL2A1, CT (Bcl-2-related Protein A1, Bcl-2-like Protein 5, Bcl2-L-5, Hemopoietic-specific Early Response Protein, Protein BFL-1, Protein GRS, BCL2L5, BFL1, GRS, HBPA1)
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumor, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5-2ug/ml Immunofluorescence: 20ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Kidney Tissue Lysate or Mouse Kidney Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 14aa from near the C-terminus of human Bfl-1. Species Sequence Homology: bovine. Epitope: located within the last 50aa
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Bfl-1. Species Crossreactivity: mouse. At least two isoforms of Bfl-1 are known to exist; this antibody will only detect the larger isoform.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.|2. Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8. 3. Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70. 4. Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem. 2000; 275:11092-9.USBio References
No references available