Technical Data

146122
Clone Type
Polyclonal
Host
Rabbit
Isotype
IgG
Grade
Affinity Purified
Applications
E
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Avian Influenza Nonstructural Protein 1 (H5N1 NS1, Nonstructural Protein 1)

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however, it is in birds that all subtypes, including the so-called avian flu or H5N1, can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. One of the less studied proteins encoded by, but not incorporated in, the influenza virus is the nonstructural protein (NS) 1. NS1 counters cellular antiviral activities and acts as a virulence factor. It can bind to double-stranded RNA and sequester it from 2-5' OAS, preventing the activation of the RNAse L, which normally acts to degrade RNA and prevent virus replication. NS1 also binds to and inhibits the anti-viral protein kinase PKR.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 14aa from the Avian Influenza Nonstructural Protein 1 at C-terminus.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Avian Influenza Nonstructural Protein 1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-186. 2. Alexander DJ. A review of avian influenza. Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on Influenza Viruses of Wild and Domestic Animals. Vet. Microbiol. 2000; 74:3-13. 3. Krug RM, Yuan W, Noah D, et al. Intracellular warfare between human influenza viruses and human cells: the role of the viral NS1 protein. Virology 2003; 309:181-9. 4. Min J-Y and Krug RM. The primary function of RNA binding by the influenza A virus NS1 protein is infected cells: inhibiting the 2-5' oligo (A) synthase/RNase L pathway. Proc. Natl. Acad. Sci. USA 2006; 103:7100-5.
USBio References
No references available
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