Technical Data

146284
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Wnt10B (Protein Wnt-10b, Protein Wnt-12, Wnt12)

Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation. Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 2-4ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Skeletal Muscle Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 15aa from human Wnt10b.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Wnt10b. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lee FS, Lane TF, Kuo A, et al. Insertional mutagenesis identifies a member of the Wnt gene family as a candidate oncogene in the mammary epithelium of int-2/Fgf-3 transgenic mice. Proc. Natl. Acad. Sci. USA 1995; 92:2268-72. 2. Nusse R and Varmus RE. Wnt genes. Cell 1992; 69:1073-87. 3. Bui TD, Rankin J, Smith K, et al. A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas. Oncogene 1997; 14:1249-53. 4. Bennett CN, Hodge CL, MacDougald OA, et al. Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells. Biochem. Biophys. Res. Commun. 2003; 302:12-16.
USBio References
No references available
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