Technical Data

146502
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-JMJD1A (Lysine-specific Demethylase 3A, JmjC Domain-containing Histone Demethylation Protein 2A, Jumonji Domain-containing Protein 1A, KDM3A, JHDM2A, JMJD1, KIAA0742, TSGA)

The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist. This antibody will not cross-react with JMJD1B or JMJD1C.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Mouse Liver Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 16aa from near the N-terminus of human JMJD1A.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human JMJD1A. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hoog C, Schalling M, Grunder-Brundell E, et al. Analysis of a murine male germ cell-specific transcript that encodes a putative zinc finger protein. Mol. Reprod. Dev. 1991; 30:173-81. 2. Sar A, Ponjevic D, Nguyen M, et al. Identification and characterization of demethylase JMJD1A as a gene upregulated in the human cellular response to hypoxia. Cell Tissue Res. 2009; 337:223-34. 3. Yamane K, Toumazou C, Tsukada Y, et al. JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor. Cell 2006; 125:483-95. 4. Knebel J, De Haro L, and Janknecht R. Repression of transcription by TSGA/Jmjd1a, a novel interaction partner of the ETS protein ER71. J. Cell Biochem. 2006; 99:319-29.
USBio References
No references available
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