Technical Data

146575
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-TRIK (Potassium Channel Subfamily K Member 18, TWIK-related Individual Potassium Channel, TWIK-related Spinal Cord Potassium Channel, TRESK, KCNK18)

TRESK (TWIK-related spinal cord K+ channel) is a two-pore domain K+ channel that induces outward rectification and functions as a background K+ channel that is abundantly expressed in the spinal cord, thymus, spleen, and leukemic T-lymphocytes. TRESK is activated by increased cytoplasmic calcium concentration through calcineurin as well as by acetylcholine and histamine. It has been suggested that TRESK is linked to acute and chronic pain by activation of calcineurin. Other studies indicate that TRESK may play a role in the regulation of T-cell function and could be an excellent target to treat T-cell originated immune dysfunction.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Rat Brain Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 17aa from human TRESK.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TRESK. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sano Y, Inamura K, Miyake A, et al. A novel two-pore K+ channel, TRESK, is localized in the spinal cord. J. Biol. Chem. 2003; 278:27406-12. 2. Han J and Kang D. TRESK channel as a potential target to treat T-cell mediated immune dysfunction. Biochem. Biophys. Res. Comm. 2009; 390:1102-5. 3. Czirjak G, Toth ZE, and Enyedi P. The two-pore domain K+ channel, TRESK, is activated by the cytoplasmic calcium signal through calcineurin. J. Biol. Chem. 2004; 279:18550-8. 4. Huang DY, Yu BW, and Fan QW. Roles of TRESK, a novel two-pore domain K+ channel, in pain pathway and general anesthesia. Neurosci. Bull. 2008; 24:166-72.
USBio References
No references available
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