Technical Data

146617
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MYBPC2 (Myosin-binding Protein C, Fast-type, Fast MyBP-C, C-protein, Skeletal Muscle Fast Isoform, MYBPCF)

Myosin binding protein C (MYBPC) is a component of the thick filament of striated muscle, with the fast-type isoform designated MYBPC2. Both the fast-type and slow-type MYBPC protein contains seven immunoglobulin C2 motifs and three fibronectin type-III repeats. MYBPC2 is typically required for strong contractions and functions under anaerobic conditions. It is more similar to the cardiac isoform (MYBPC3) than to the slow-type isoform (MYBPC1) in terms of pCa50-indexed force development, length-independent cooperativity and length dependent activation. It has been suggested that in cardiac and fast muscle MYBPC2 contributes to an internal load, possibly by binding to actin via its N-terminal region.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Mouse Skeletal Muscle Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 15aa from near the C-terminus of human MYBPC2.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MYBPC2. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Weber FE, Vaughan KT, Reiach FC, et al. Complete sequence of human fast-type and slow-type muscle myosin-binding-protein C (MyBP-C). Differential expression, conserved domain structure and chromosome assignment. Eur. J. Biochem. 1993; 216:661-9. 2. Konhilas JP, Irving TC and De Tombe PP. Length-dependent activation in three striated muscle types of the rat. J. Physiol. 2002; 544:225-36. 3. Flashman E, Korkie L, Watkins H, et al. Support for a trimeric collar of myosin binding protein C in cardiac and fast skeletal muscle, but not in slow skeletal muscle. FEBS Lett. 2008; 582:434-8.
USBio References
No references available
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