Rabbit Anti-EFHD2 (EF Hand Domain Containing Protein D2, MGC4342, RP3-467K16.3, Swiprosin 1, Swiprosin-1, SWS1)
EFHD2, also known as Swiprosin-1 or SWS1, is an EF-hand and coiled-coil-containing adaptor protein that plays a role in lymphocyte physiology. EFHD2 exhibits the highest expression in CD8+ T cells and immature B cells. It provides a membrane scaffold that is required for the Syk-, SLP-65-, and PLCgamma2-dependent B-cell receptor (BCR)-induced calcium flux. EFHD2 may also regulate BCR-induced immature and primary B-cell apoptosis. It controls spontaneous apoptosis through the regulation of BCL2L1 abundance. Also, EFHD2 plays a role as negative regulator of the canonical NF-kappa-B-activating branch.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Mouse Brain Tissue Lysate and Human Brain Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 16aa from near the N-terminus of human EFHD2.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human EFHD2. Species Crossreactivity: mouse and rat.
