Technical Data

146638
Clone Type
Polyclonal
Host
Chicken
Source
Human
Isotype
IgY
Grade
Affinity Purified
Applications
E IC IF WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Chicken Anti-WDR40A (DCAF12, KIAA1892, TCC52, WDR40A, DDB1-and CUL4-associated Factor 12, Centrosome-related Protein TCC52, Testis Cancer Centrosome-related Protein, WD Repeat-containing Protein 40A)

Cancer-testis (CT) antigens are a type of antigen whose expression is highly restricted, primarily in normal testis, but aberrantly in different tumor types. Their immunogenicity in cancer patients makes them prime targets for immunotherapy of human tumors. One such CT antigen is TCC52, a novel centrosomal protein. TCC52 is a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. The normal biological function of this protein is not known at this time.

Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
293 Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 15aa from near the C-terminus of human TCC52.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TCC52. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Scanlan M, Simpson A, and Old L. The cancer/testis genes: review, standardization, and commentary. Cancer Immun. 2004; 4:1-15. 2. Xia L, Li Y, Yang D, et al. Identification of new centrosome proteins by autoimmune patient sera. Sci. China C. Life Sci. 2007; 50:194-202. 3. Li S, Hu X, Cui S, et al. Novel centrosome protein, TCC52, is a cancer-testis antigen. Cancer Sci. 2008; 11:2274-9.
USBio References
No references available
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