Technical Data

146692
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IC IF WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ZC3HAV1 (Zinc Finger CCCH-type Antiviral Protein 1, ADP-ribosyltransferase Diphtheria Toxin-like 13, ARTD13, Zinc Finger CCCH Domain-containing Protein 2, Zinc Finger Antiviral Protein, ZAP, ZC3HDC2, PRO1677)

The zinc finger antiviral protein (ZC3HAV1) is a CCCH type zinc finger protein that induces an innate immunity to infections by retrovirus by preventing the accumulation of viral RNAs in the cytoplasm and recruits the RNA processing exosome to degrade target RNAs, thereby inhibiting virus replication. ZC3HAV1 is localized in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. ZAP is a direct target gene of IRF3 action in cellular antiviral responses.

Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
HeLa Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 14aa from near the C-terminus of human ZC3HAV1.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ZC3HAV1

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Gao G, Guo X, and Goff SP. Inhibition of retroviral RNA production by ZAP, a CCCH-Type zinc finger protein. Science2002; 297:17036. 2. Guo X, Ma J, Sun J, et al. The zinc-finger antiviral protein recruits the RNA processing exosome to degrade the target mRNA. Proc. Natl. Acad. Sci. USA2007; 104:1516. 3. MacDonald MR, Machlin ES, Albin OR, et al. The zinc finger antiviral protein acts synergistically with an interferon-induced factor for maximal activity against alphaviruses. J. Virol. 2007; 81:13509-18. 4. Wang N, Dong Q, Li J, et al. Viral induction of the zinc finger antiviral protein is IRF3-dependent but NF-kappaB-independent. J. Biol. Chem.2010; 285:6080-90.
USBio References
No references available
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