Rabbit Anti-ATP11B (Probable Phospholipid-transporting ATPase IF, ATPase IR, ATPase Class VI Type 11B, ATPIF, ATPIR, KIAA0956)
ATP11B is a widely expressed integral membrane ATPase and is thought to drive the transport of phospholipids across membranes. It has been suggested that ATP11B is hormonally regulated and plays a role in the subnuclear trafficking of transcription factors with RING motifs. While the exact molecule ATP11B transports is unknown, increased mRNA expression of the homologous ATP11A has been observed in cells resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs), suggesting that ATP11B may also play a role in cell survival under harsh conditions.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunocytochemistry: 10ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
K562 Cell Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 19aa from near the N-terminus of human ATP11B.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ATP11B. Species Crossreactivity: mouse.
