Technical Data

146707
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-HVCN1 (Voltage-gated Hydrogen Channel 1, Hydrogen Voltage-gated Channel 1, HV1, Voltage Sensor Domain-only Protein, VSOP, UNQ578/PRO1140)

HVCN1 (hydrogen voltage-gated channel 1), a voltage gated proton channel protein that is highly expressed in immune tissues, mediates the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing. HVCN1 moderates the voltage-dependent proton permeability of excitable membranes by allowing the flow of protons in accordance to their electrochemical gradient. HVCN1 is sensitive to zinc ions, and can be inhibited by them. HVCN1 may represent a new therapeutic target for B cell malignancies on continued signaling via the B cell antigen receptor.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.5-1ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human Spleen Tissue Lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 17aa from near the C-terminus of human HVCN1.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human HVCN1. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ramsey IS, Moran MM, Chong JA, et al. A voltage gated proton-selective channel lacking the pore domain. Nature2006; 440:1213-6. 2. Sasaki M, Takagi M, and Okamura Y. A voltage sensor-domain protein is a voltage-gated proton channel. Science2006; 312: 589-92. 3. Morgan D, Capasso M, and Musset B. Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis. Proc. Natl. Acad. Sci. USA2009; 106:18022-7. 4. Capasso M, Bhamrah MK, Henley T, et al. HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species. Nat. Immunol.2010; 11:265-72.
USBio References
No references available
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